CHOK1细胞​

CHOK1细胞

1957年，PuckTT从成年中国仓鼠卵巢的活检组织建立了CHO细胞，CHOK1细胞是CHO的一个亚克隆。CHOK1细胞的生长需要脯氨酸。 

细胞名称：仓鼠卵巢细胞

细胞简称：CHO-K1

细胞别称：CHO K1; CHOK1; CHO cell clone K1; GM15452

产品货号：TCO-C005

规格： 1X10^6

种属来源：仓鼠

组织来源：卵巢

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：F12K+10%FBS+1%P/S

配套培养基货号：TCO-G005

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

  CHOK1细胞为转化细胞系，   在工业生产上应用较多，  CHOK1细胞 染色体分布频率是2n=22，系亚二倍体细胞。海星细胞库保存CHO-K1细胞株，编号为TCO-C005，被广泛地用于重组DNA蛋白的表达。

参考文献：

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365